The trip comes after the detention of two Canadians by Chinese authorities.
The train with 40 refrigerated-containers is 620 meters long. It carried about 1,160 tonnes of concentrate juice from the border port of Horgos to Hangzhou.
The two Orange Line cars were made at China Railroad Rolling Stock Corp's factory in Springfield, Massachusetts.
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The two started their historic summit on Tuesday morning at the Capella hotel in Sentosa on an optimistic note.
The tunnel, which links the Hong Kong Island and Kowloon, has been shut since Nov 13, when rioters went on a rampage, throwing petrol bombs, setting fire to toll booths and a nearby footbridge, said Chief Secretary for Administration Matthew Cheung.
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The two sides also have conducted constructive talks on further expanding trade in manufactured goods and services, better protecting intellectual property rights and encouraging two-way investment.
The two are seeking to gain ground against former Vice President Joe Biden, who currently leads in opinion polls among Democratic contenders.
The two were charged with allegedly violating campaign finance law by funneling foreign money to US politicians. They reportedly helped Giuliani seek evidence of Democratic presidential candidate Joe Biden's alleged involvement in a corruption case in Ukraine.
The two pieces of land, which are in Pudong New Area's Zhangjiang area and Jiading district, were sold on the same date to Shanghai Zhangjiang (Group) Co Ltd and Shanghai Jiading New City Development Co Ltd, respectively, both state-owned companies, the Shanghai Observer reported.